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Cell Membrane --- Chemistry Techniques, Analytical --- ultrastructure --- physiology --- methods --- physiology. --- ultrastructure. --- methods. --- Membranes (Biology) --- Cell membrane --- Chemistry --- Physiology --- Ultrastructure --- Analytical --- Methods --- Membranes (Biology). --- Physiology. --- Ultrastructure. --- Methods. --- Cell Membrane - ultrastructure --- Cell Membrane - physiology --- Chemistry Techniques, Analytical - methods
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Current techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field.
Biophysical Phenomena --- Chemistry Techniques, Analytical --- Diagnostic Imaging --- Macromolecular Substances --- methods --- chemistry --- Biophysics. --- Molecular biology. --- Biophysical Phenomena. --- Diagnostic Imaging. --- methods. --- chemistry. --- Biophysique --- Biologie moléculaire --- Biophysical phenomena. --- Chemistry techniques, Analytical --- Diagnostic imaging. --- Macromolecular substances --- Biophysique. --- Biologie moléculaire. --- Chemistry Techniques, Analytical - methods --- Macromolecular Substances - chemistry
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Analytical chemistry --- Chemistry, Analytic --- Chimie analytique --- Chemistry, Analytical --- Chemical apparatus --- Chimie --- methods --- Appareils et matériel --- Chemistry Techniques, Analytical --- 543.08 --- #WSCH:AAS1 --- Apparatus, Chemical --- Chemical instruments --- Chemistry --- Physical instruments --- Scientific apparatus and instruments --- Analysis, Chemical --- Chemical analysis --- Metallurgical analysis --- Mineralogy, Determinative --- Analytical measurement --- methods. --- Apparatus --- Instruments --- Instrumentele analyse --- Instrumentele analyse. --- 543.08 Analytical measurement --- Appareils et matériel --- Analytical chemistry. --- Analytic chemistry --- Chemistry Techniques, Analytical - methods --- Instrumentation
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Thromboxanes --- Eicosanoic Acids --- Chemistry Techniques, Analytical --- Prostaglandins --- Immunoassay --- Lipoxygenase --- analysis --- methods --- 577.175.859 --- 57.088 --- methods. --- analysis. --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 577.175.859 Prostaglandins --- Thromboxanes - analysis --- Eicosanoic Acids - analysis --- Chemistry Techniques, Analytical - methods --- Prostaglandins - analysis --- Immunoassay - methods --- Lipoxygenase - analysis
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Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions.
Protein binding --- Ligands --- Proteins --- Chemistry Techniques, Analytical --- Protein Binding --- chemistry --- methods --- Chemistry, Analytic -- Laboratory manuals. --- Protein binding -- Laboratory manuals. --- Laboratory Chemicals --- Specialty Uses of Chemicals --- Binding, Protein --- Chemistry, Analytic --- Biochemistry --- Allosteric proteins --- Radioligand assay --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Protein binding - Laboratory manuals --- Ligands - laboratory manuals --- Proteins - chemistry - Laboratory Manuals --- Chemistry Techniques, Analytical - methods - laboratory manuals --- Protein Binding - Laboratory Manuals
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In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.
moleculaire biologie --- Molecular biology --- DNA (deoxyribonucleic acid) --- flowcytometrie --- Methodology --- Chemistry techniques, Analytical -- Methods. --- Genetic techniques. --- Molecular biology -- Methodology. --- Molecular diagnosis -- Methodology. --- Molecular Biology --- Cytological Techniques --- Genetic Techniques --- Investigative Techniques --- Clinical Laboratory Techniques --- Biochemistry --- Genetics --- Biology --- Biological Science Disciplines --- Chemistry --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- 57.088 --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Methodology. --- Cytology. --- Molecular biology - Methodology
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Applied chemical analysis --- Materials sciences --- Materials --- Chemistry, Analytic --- Materials Testing --- Chemistry Techniques, Analytical --- Testing --- methods --- 620.18 --- -Engineering --- Engineering materials --- Industrial materials --- Engineering design --- Manufacturing processes --- Analysis, Chemical --- Analytical chemistry --- Chemical analysis --- Metallurgical analysis --- Mineralogy, Determinative --- methods. --- Investigation of structure of materials. Metallography. Analogous study of non-metals --- Chemistry, Analytic. --- Testing. --- -methods. --- 620.18 Investigation of structure of materials. Metallography. Analogous study of non-metals --- -Analysis, Chemical --- Engineering --- Analytic chemistry --- Chemistry --- Computer software. --- Electrochemistry. --- Materials - Testing --- Materials Testing - methods --- Chemistry Techniques, Analytical - methods --- Chromatography --- Polymers --- Spectroscopy --- Surface (chemistry and physics) --- Viscosity
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